La cytométrie en flux
(2° Éd.)
Jean-François MAYOL, Corinne LAPLACE-BUILHÉ, Christian D. MULLER
Lavoisier / Tec & Doc
Résumé :
La cytométrie en flux, technique permettant l’analyse rapide des cellules, a considérablement évolué ces dernières décennies et a contribué à de nombreuses avancées scientifiques et médicales. Ses avancées ont ouvert la porte à des applications devenues incontournables dans différentes spécialités telles que l’hématologie, l’immunologie, la microbiologie, la biologie végétale et marine. La transversalité interdisciplinaire de la cytométrie en flux en fait toute la richesse.
La deuxième édition de La Cytométrie en Flux fait le point, en 29 chapitres, sur toutes les nouveautés dues aux avancées instrumentales, à la miniaturisation des lasers, aux progrès de l’optique, de la fluidique mais aussi de l’informatique, des logiciels embarqués et de l’analyse de données, sans oublier les réactifs couvrant maintenant une gamme inimaginable il y a seulement encore quelques années. De nombreux thèmes sont ainsi abordés :
- Principes, techniques, statistiques appliquées à la cytométrie
- Applications dans différents domaines (hématologie, immunologie, détection des microparticules, étude des cellules souches, pharmacologie anticancéreuse…)
- Criblage à haut débit (high throughput screening), applications du fluorescent cell barcoding
- Assurance qualité, accréditation
- Avancées de la cytométrie couplée à d’autres techniques comme l’analyse spectrale et la spectrométrie de masse
- Gestion des plateformes
- Hygiène et sécurité
Les nombreuses illustrations en couleurs et les protocoles d’analyse viennent compléter ce livre didactique. Cet ouvrage est destiné aux enseignants et étudiants, chercheurs, biologistes, cliniciens, ingénieurs, techniciens et industriels désireux d’approfondir ou de mettre à jour leurs connaissances. Il permettra surtout aux utilisateurs non experts d’acquérir les concepts de base de la cytométrie en flux.
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